Evaluation of food-drug interaction potential of Aegle marmelos (L.) Corrêa through metabolism mediated cytochrome P450 inhibition assay

In the present study, possible cytochrome enzyme inhibition potential of Aegle marmelos (L.) Corrêa fruit and its bioactive compound was evaluated. Interaction potential of Aegle marmelos extract was evaluated through CYP450carbonmonoxide complex (CYP450-CO) assay. Additionally, influence on individual major human cytochrome P450 drug metabolizing enzymes such as CYP3A4, CYP2D6, CYP2C9 and CYP1A2 were studied through fluorescence screening assay. Bioactive compound was quantified through RP-HPLC, in order to standardize the sample material. Aegle marmelos extract showed higher IC50 value than the respective standard inhibitors and lower than the bioactive compound imperatorin. Aegle marmelos extract showed significantly lesser (P<0.001, P<0.01) interaction potential than the standard inhibitors. The research findings indicated that selected sample is unlikely to cause clinically relevant drug interactions involving inhibition of major CYP isozymes.